On the study of the antiviral activity of individual probiotic strains of lacto- and bifidobacteria

19  07.2021 Scientists of the Institution of epidemiology and microbiology named after Academician IN Blokhina of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare studied the antiviral activity of six industrial probiotic strains.

Currently, the world scientific community is of great interest to the question of the interaction of probiotic bacteria of the genera Lactobacillus and Bifidobacterium with viruses, in connection with their use for the prevention and treatment of viral infections.
Scientists of the Institution of epidemiology and microbiology named after Academician IN Blokhina of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare studied the antiviral activity of six industrial probiotic strains - L. plantarum 8 RA 3, L. fermentum 39, L. fermentum 90 TC-4, B. bifidum 1, B. bifidum 791 and B. longum 379, which form the microbial basis of liquid probiotic dietary supplements for food of the LB-complex group ("LB-complex", "LB-complex L", "LL-complex"), developed and produced at the institute (certificates of state registration RU .77.99.88.003.E.000946.01.15, RU.77.99.88.003.E.002522.06.18; RU77.99.88.003.R.003638.10.19).
Employees of the Institution of epidemiology and microbiology carried out work to study the interaction of L. plantarum 8 RA 3 and L. fermentum 39 strains with rotaviruses. It has been established that L. fermentum 39 cells are capable of adsorbing rotavirus particles on themselves, which makes it possible to scientifically substantiate a new aspect of the use of probiotics, including this strain, in acute gastroenteritis caused by human rotaviruses.
The results of the research carried out were formalized in a scientific article, which was published in the journal "Microbiology": Solovyova I.V., Novikova N.A., Tochilina A.G., Belova I.V., Kashnikov A.Yu., Sashina T.A. ., Zhirnov V.A., Molodtsova S.B. Probiotic strain Lactobacillus fermentum 39: biochemical properties, genome features, antiviral activity // Microbiology. - 2021. - T. 90, No. 2. - P. 215–222. (DOI: 10.31857 / S0026365621020142).
Employees of the Institution of epidemiology and microbiology, together with scientists from the State Scientific Center for Virology and Biotechnology "Vector" of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Novosibirsk), analyzed the complete genome of probiotic strains L. plantarum 8 RA 3, L. fermentum 90 TC-4, L. fermentum 39 , B. bifidum 791, B. bifidum 1, B. longum 379 and study of their activity against viruses of highly pathogenic influenza A - A / Lipetsk / 1V / 2018 (H1N1 pdm09) (EPI_ISL_332798), A / common gull / Saratov / 1676 / 2018 (H5N6) (EPI_ISL_336925) and SARS-CoV-2 virus strain Australia / VIC01 / 2020 (GenBank: MT007544.1). As a result, it was confirmed that the antiviral activity is a strain-specific trait, and it was found that the strains L. fermentum 39, L. fermentum 90 TC-4 and B. bifidum 791 exhibit pronounced activity against influenza A viruses in MDCK cells. Activity against SARS-CoV-2 virus was demonstrated only by L. fermentum strain 90 TC-4 in VERO cells. Thus, it was proved that the strains L. fermentum 39, L. fermentum 90 TC-4 and B. bifidum 791 have a pronounced antiviral activity, and probiotics containing these strains can be used to prevent diseases caused by rotaviruses, influenza A viruses and the SARS-CoV-2 virus.
It is advisable to use medicines and dietary supplements containing these strains in complex programs for the treatment and rehabilitation of patients with viral infections. The results of the research were formalized in a joint scientific article, which was published in the foreign journal "Biomed Research International" Soloveva IV, Ilyicheva TN, Marchenko V.Yu., Pyankov OV, Tochilina AG, Belova IV, Zhirnov VA, Bormotov NI, Skarnovich MO, Durymanov AG, Molodtsova SB, Filippova EI, Ovchinnikova AS, Magerramova AV, Ryzhikov AB, Maksyutov RA Genome Features and In Vitro Activity against Influenza A and SARS-CoV-2 Viruses of Six Probiotic Strains // BioMed Research International. - 2021. - V. 2021. - ID 6662027, 11 p. (https://doi.org/10.1155/2021/6662027).

Currently, the joint work of Institution of epidemiology and microbiology and State Scientific Center of Virology and Biotechnology is to study the antiviral activity of production-promising probiotic strains of lactobacilli isolated from healthy people of various age groups, from the collection of the Institution of epidemiology and microbiology against rotaviruses, noroviruses, highly pathogenic influenza viruses and various genovariants of the SARS-CoV-2 virus.